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DBTSS is a database which contains precise positional information for transcription start
sites (TSSs) of eukaryotic mRNAs. In this update, we included additional 328 million TSS tag
data, which were generated by massively sequencing analysis of the full-length cDNAs in
humans and mice. The TSS tags were collected from a total of 33 different cell types or
cultured conditions. Also, differential usages of the TSSs depending on the cellular
circumstances are made retrievable and viewable by a series of newly implemented interfaces.
The retrieved promoter information is further linked to the comparative genomics viewer to
study evolutional turnover of the TSSs. By accommodating unprecedented amount of TSS data,
the updated DBTSS represents, for the first time, the dynamically changing nature of the TSSs
of mammalian genes, depending on cellular circumstances and in the course of evolution,
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