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In order to overcome the difficulty described in the
previous section, we developed a new method to introduce a "sequence
tag" at the 5'-end, which we call the "Oligo-Capping"
method. This method allows us to replace the cap structure of mRNA
with the synthetic oligo-nucleotide enzymatically. Each mRNA product
of the "Oligo-Capping" contains the "sequence tags" at the both
ends, which is polyA at the 3'-end and the cap-replaced oligo at
the 5'-end. With "Oligo-Capped" mRNA as a starting material, a new
system is developed to selectively clone the cDNA which contain
both of the sequence tags at the respective ends. Following the
scheme shown in Figure 1, a cDNA library is constructed in which
the content of "full-length" cDNA is significantly enriched ("full
length-enriched" cDNA library).
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