DBTSS; DataBase of Transcriptional Start Sites.

To support transcriptional regulation studies, we have constructed the DBTSS, which represents exact positions of transcriptional start sites (TSSs) in the genome based on our unique experimentally validated TSS sequencing method, TSS-seq. This database includes DBTSS data of a major part of human adult and embryonic tissues are covered. DBTSS now contains 491 million TSS tag sequences for collected from a total of 20 tissues and 7 cell cultures. We also integrated our newly generated RNA-seq data of subcellular- fractionated RNAs and ChIP-seq data of histone modifications, RNA polymerase II and several transcriptional regulatory factors in cultured cell lines. We also included recently accumulating external epigenomic data, such as chromatin map of the ENCODE project.

Taken together, we provide a unique data resource to investigate what genomic features are observed in a particular genomic coordinates in a wide variety of samples. We believe new DBTSS is helpful to understand biological consequences of the massively identified TSSs and identify human genetic valuations which are associated with disordered transcriptional regulations.

Funding:

DBTSS is supported by JSPS under the grant ID 221S0002

Ayako Suzuki, Shin Kawano, Toutai Mitsuyama, Mikita Suyama, Yae Kanai, Katsuhiko Shirahige, Hiroyuki Sasaki, Katsushi Tokunaga, Katsuya Tsuchihara, Sumio Sugano, Kenta Nakai, Yutaka Suzuki. DBTSS/DBKERO for integrated analysis of transcriptional regulation. Nucleic Acids Res. 2018 (Database issue).

Suzuki A, Mimaki S, Yamane Y, Kawase A, Matsushima K, Suzuki M, Goto K, Sugano S, Esumi H, Suzuki Y, Tsuchihara K. Identification and characterization of cancer mutations in Japanese lung adenocarcinoma without sequencing of normal tissue counterparts. PLoS One. 2013 Sep 12;8(9).

Yamashita R, Sathira NP, Kanai A, Tanimoto K, Arauchi T, Tanaka Y, Hashimoto S, Sugano S, Nakai K, Suzuki Y. (2011) Genome-wide characterization of transcriptional start sites in humans by integrative transcriptome analysis. Genome Res. 2011 Mar 3.

Tsuchihara K, Suzuki Y, Wakaguri H, Irie T, Tanimoto K, Hashimoto S, Matsushima K, Mizushima-Sugano J, Yamashita R, Nakai K, Bentley D, Esumi H and Sugano S. (2009) Massive transcriptoional start site analysis of human genes in hypoxia cells. Nucleic Acids Res. 2009 Feb 22.

[Japanese]
"Database Manual" (Yodosha)