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The full-length cDNA, which contains the entire sequence
of the mRNA, is the ultimate goal for the cDNA cloning. Unfortunately,
cDNA libraries constructed by many types of conventional methods
have high content of non-full length cDNA clones. One of the reasons
for this high content is that reverse transcriptase tends to stop
during the first strand synthesis and falls off, leaving non-full
length cDNA. Thus, non-full length cDNA is an inevitable result
of the use of reverse transcriptase for the cDNA synthesis.
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