1-1-3. Construction of A Full-length cDNA Library

In order to overcome the difficulty described in the previous section, we developed a new method to introduce a "sequence tag" at the 5'-end, which we call the "Oligo-Capping" method. This method allows us to replace the cap structure of mRNA with the synthetic oligo-nucleotide enzymatically. Each mRNA product of the "Oligo-Capping" contains the "sequence tags" at the both ends, which is polyA at the 3'-end and the cap-replaced oligo at the 5'-end. With "Oligo-Capped" mRNA as a starting material, a new system is developed to selectively clone the cDNA which contain both of the sequence tags at the respective ends. Following the scheme shown in Figure 1, a cDNA library is constructed in which the content of "full-length" cDNA is significantly enriched ("full length-enriched" cDNA library).
 
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