完全長cDNAの構築

2-1. 従来のcDNA作成技術の欠点

2-2. 完全長cDNAのクローニング

2-3. 完全長cDNA libraryの作成

2-4. 実験手法

References 完全長cDNAの構築

Maruyama K, Sugano S. (1994)
Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene 138: 171-174.

Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S. (1997)
Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. Gene 200: 149-156.
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